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KMID : 0880520070430020073
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Chonnam Medical Journal
2007 Volume.43 No. 2 p.73 ~ p.79
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Protective Effects of Epigallocatechin-3-gallate against 1-methyl-4-phenylpyridinium-induced Dopaminergic Neuronal Cell Death
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Noh Hai-Ghuen
Jang Su-Jeong
Park Sung-Jun
Kim Song-Hee
Jeong Han-Seong
Park Jong-Seong
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Abstract
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1-methyl-4-phenylpyridinium (MPP+) is a neurotoxin that selectively inhibits the mitochondrial functions of dopaminergic neurons in the substantia nigra pars compacta. MPP+ administration is accepted as in vivo and in vitro model for Parkinson’s disease (PD). In the present study, we found that MPP+ induced a concentration-dependent decrease in SN4741 mouse dopaminergic cell viability. Even though epigallocatechin-3-gallate induced a suppression of the MPP+-induced apoptosis as well as an increase of the cellular proliferation, the mechanisms underlying this process have not to be completely clarified yet. We have investigated whether EGCG plays a neuroprotective role by activating cell survival systems such as protein kinase C, and acting on the anti-apoptotic and the pro-apoptotic genes in SN4741 dopaminergic cells. EGCG restored the reduced phospho-PKC activities caused by MPP+ toxicity. In addition, gene expression analysis revealed that EGCG prevented both the MPP+-induced expression of the pro-apoptotic gene mRNA, bad and bax, and the decrease of the anti-apoptotic gene mRNA, bcl-2 and bcl-.Xl. These results suggest that the neuroprotective mechanism of EGCG against MPP+-induced apoptotic cell death includes stimulation of PKC and modulation of cell survival and death genes
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KEYWORD
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EGCG, Neuroprotection, 1-methyl-4-phenylpyridinium, Parkinson’s disease, Dopaminergic cells
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